Possible Cause
Solution
| Active sites on column packing | Use mobil-phase modifier, competing base (basic compounds), or increase buffer strength; use higher coverage column packing. |
| Column overloaded with sample | Decrease sample amount or use larger-diameter column |
| Increasing flow rate | Check and reset pump flow rate. |
| Loss of bonded stationary phase or base silica | Use mobile-phase pH between pH 2 and pH 8 |
| Varying column temperature | Thermostat or insulate column; ensure laboratory temperature is constant. |