| Autosampler flow lines blocked | Check flow and clear any blockages |
| Detector attenuation set too high | Reduce detector attenuation |
| First few sample injections - sample adsorption in injector sample loop or column | Condition loop and column with concentrated sample. |
| Injector sample loop underfilled | Overfill loop with sample |
| Not enough sample injected | Increase amount of sample inected |
| Peaks are outside detector's linear range | Dilute or concentrate sample to bring detector
response into linear range |
| Sample losses during sample preparation | Use internal standard during sample prepartion; optimize sample preparation method |
| Sample losses on column | See problem: Recovery |