Quiz
1) True or false: Throughput is critical in applications such as analytical HPLC.2) Which of the following methods of column efficiency calculation is the most sensitive to "tailing"?

	a)	Moment
	b)	Three-sigma
	c)	Half-peak height
	d)	Tangent

3) Predict the order of elution of the following compounds using a C18 µBondapack reversed-phase column using 200 mM ammonium formate, pH 9 as the mobile phase:

	1.	Linoleic acid
	2.	Arachidic acid
	3.	Oleic acid
	4.	Palmitic acid

4) The ability of a compound to bend light is called its ____________, while its ability to absorb and re-emit light is called its ____________.

	a)	turbidimetry, absorption
	b)	refractive index, fluorescence
	c)	nephelometry, ionization
	d)	oxidation, reduction

5) Predict the order of elution of the following sequences using an SCX (strong cation-exchange) column using 500 mM potassium phosphate, pH 4 as the mobile phase:

	1.	His-Arg-Lys-Pro
	2.	Met-Gly-Gly-Ile
	3.	Asp-Glu-Val-Ala
	4.	Phe-Thr-Thr-Cys

6) Which of the following is/are true?

	I.	Bandwidth and resolution are determined by different factors in isocratic and gradient elution
	II.	Advantages of gradient elution include nearly equal bandwidths and improved resolution
	III.	Size-exclusion and affinity chromatography are two techniques utilized in polytyptic chromatography
	a)	I only
	b)	I & II
	c)	I, II, & III
	d)	II & III

Match the following chromatographic procedure with its mechanism-of-action/binding technique:

_____	7)	Reversed-phase		a.	Molecular weight
_____	8)	SEC		b.	Hydrophilic ity
_____	9)	IEC		c.	Hydrophobic ity
_____	10)	Normal phase		d.	Protonation/Ionization

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